There is a book on this subject from Timber Press. I have a friend who took a course and, being a microbiologist he was able to simplify the process. He did not use a glove box but rather an empty aquarium placed on its side. He used bleach and alcohol in separate misting bottles and had very high rates of success, doing micropropagation of haworthias and cactus. He said that a hood or glove box would have made little difference in rate of contamination. He wrote an article that was published in the Cactus Journal--author, Michael Louie.

The link below gives a picture of my set up.

Here is a link that might be useful: my set up

Try ebay under Bussines & Industrial, type in laminar flow hood or if you want to build your own type in hepa.

Rob,
What kind of car do you need the hood and glove box for?
I may have them at my Junkyard.

just joshing....

Best Regards,
Bruce (Junkmanme)
P.S. I forgot...I owe you some "Big Jim" anaheim seeds.

Back in the early 70s when I was into hybridizing, and tissue culturing orchids, I used to use a large plastic bag. I found a source for clear polyethylene bags about 2 foot by 3 foot, cut the lower corners off, and taped the cuffs of a pair of playtex gloves to the openings with the gloves pointing into the bag. Voila!!! A glove bag. Worked fine for both seed and meristem culture. You can sterilize with diluted bleach, or put the whole thing under a UV light, although this degrades the plastic rather rapidly.

George