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Help propagating tomatoes from tissue culture

10 years ago
last modified: 10 years ago

I have recently gotten into plant tissue culture propagation after watching some videos by Bill Graham. I've been using the kits available from Monster Gardens, which are designed to make the process very simple for the amateur hobbyist. I have had a lot of fun, and some great success with lots of different plants, including strawberries, bananas, irises, and so-on.

One plant that I have not had luck with, however, are tomatoes. When put into the multiplication medium, it quickly forms a lot of lumpy callus, versus branching like most other plants I've used. I can propagate tons of the callus, but I cannot get it to ever form plants again. I have used their root medium, but it doesn't stimulate plants to form from the callus (the callus just continues to expand like in the multiplication medium).

I assume the kits from Monster Gardens probably just don't have the right mixture of hormones for tomatoes. The kits are excellent for beginners (even my six year old son enjoys doing it), but with only three different kits to choose from, they don't provide much flexibility.

I've done some googling on the subject, but it seems outside of Monster Gardens, most information about tissue culture gets very technical very quick, and many of the components can be a bit pricey. I thought before I start going too crazy with the experimentation, I would see if anyone has had some experience with propagating tomatoes from tissue culture, or maybe some advice on things to try to get the callus to form plants again.

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