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A German study testing for Rose Rosette Virus

9 years ago

Google translation of a German paper presented at a meeting.

"Infection of roses with viruses with special attention to the Rose rosette virus and Ilarviren

Virus-suspicious symptoms such as checks, chlorotic ring spots and line patterns, partly in combination with growth spills and death-related symptoms, have been observed for several years in the rosaceae of a varietal collection. The cause is a variety of plant viruses, which can infect roses. Various viruses (Tomato ringspot virus, Tomato ringspot virus, Tirolo mosaic virus, Tomato ringspot virus) are commonly found as virus complexes in different combinations Roses are most widespread (Milleza et al., 2013). In Germany, the Raspberry ringspot nepovirus (RpRSV) was recently detected in roses of Mainau Island (Bargen et al., 2015). In addition, other viruses have been described in roses, including the Rose rosette virus (RRV, genus Emaravirus), which is widely distributed in the United States, causing considerable loss of yield in all areas of rosaceae cultivation (Babu et al., 2016). Leaf samples of various varieties including tea hybrids, climbing roses, foribunda or polyanthoses with the above-mentioned symptoms were examined for a virus infection by means of DAS-ELISA or RT-PCR method using genus-specific oligonucleotides in connection with sequencing of the PCR products. In the study of 8 rose samples of different varieties with virus-suspicious leaf symptoms, infection with RRV could be detected by the use of generic (Elbeaino et al., 2013) as well as by RRV-specific oligonucleotides (Laney et al RT-PCR can be excluded. Further results of this study on the detection of Ilarviruses are presented and discussed.

http://www.schoenmuth.de/Posterdok/Posterdokumentation-PM2016/2016-PST-Halle/2016-PST-Posterabstracts/Posterabstract-PST2016-122a-Stummer_28z.pdf

A second link indicates that no rose rosette virus was found.

Again from a Google translation:

"For a number of years, a variety of virus-suspicious symptoms have been observed in the rose stands of a rose collection in Germany. They are manifested by checks, chlorotic ring spots, and lines (Fig. 1), partly in connection with growth spills and deaths. A variety of plant viruses are to be considered for the infection of roses, a. Various ilarviruses such as, For example, Apple mosaic virus (ApMV), Prunus necrotic ringspot virus (PNRSV) or Tobacco streak virus (TSV) (Milleza et al., 2013). Furthermore, the Raspberry ringspot nepovius (RpRSV) was recently found on the island of Mainau in Germany.
(Bargen et al., 2015). Another major virus that infects roses is the rose rosette emaravirus (RRV). It is widespread in the United States and leads there to considerable losses in income in all areas of the cultivation of roses (Babu et al., 2016). The aim of this study was to detect plant viruses, which could be the cause of the observed disease symptoms on the roses. The emphasis was on the detection of Ilar- and Potyviren as well as of RRV.
Ilarviren
Detection of PNRSV and potyviruses (Table 1) in the ELISA • Confirmation Ilarvirus infection with RT-PCR (Fig. 2) • No detection of potyviruses with RT-PCR • Identification of ApMV in "Pelé" and PNRSV types in the tea hybridomas " Promise "," Climbing New Yorker "and" Parade "by sequencing selected products of the Ilarvirus PCR • Exclusion of the infection with RRV of 8 rose samples of different varieties with chlorotic ring spots, graying or oak leaf patterns as no products in either category or RRV Specific PCR • No detection of the tested viruses in several symptom bearing roses"

Also see Table 1.

http://www.schoenmuth.de/Posterdok/Posterdokumentation-PM2016/2016-PST-Halle/2016-PST-Posters/Stummer-DPST2016-Poster.pdf