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DNA Matching Service???

18 years ago

I've seen posts here referring to DNA matching at UC Davis. Does anyone know if they will, for a fee or otherwise, run a DNA match on a cutting sent to them?

Cattlemen can send blood samples from a calf and the bulls in a given pasture to a lab for tests to determine paternity. It would be helpful if we could send it cuttings and identify our unknown figs.

Ox

Comments (13)

  • 18 years ago

    The short answer is "no".

  • 18 years ago

    Rats:
    Someone should put a bug in their ear. If they offered this service for a modest fee they would get lots of business and keep a couple or more undergrads busy in their spare time while learning to do DNA work. They are probably the only place in the country with any fig ID data.
    Ox

  • 18 years ago

    I agree Ox. I would pay for such a service also. Maybe we should take a poll and petition them.

  • 18 years ago

    It will not be cheap , modest, or reasonable.

  • 18 years ago

    it would go a long way in eliminating some confusion in typing figs. But we all have so much fun trying to guess.

  • 18 years ago

    Pitangadiego: I see no reason that it should be all that expensive if you really want to know what you have.

    Best I recall the cattle labs do DNA tests for about $35--I'll check that in the next day or so. Since there are a limited number of Fig cultivars the lab would simply have to sequence the test subject, then run a computer match against its library of known fig varieties.

    Cattle labs have to sequence the test subject and from one to several possible sires, so fig testing should be less expensive.

    But then, what do I know?
    Ox

  • 18 years ago

    CSI for figs, cool!

  • 18 years ago

    It seems that ISHA has done some type of DNA research on 30 types to read the complete abstract you have to become a member here is a brief that is on there site,
    ISHS Acta Horticulturae 598: International Symposium on Sustainable Use of Plant Biodiversity to Promote New Opportunities for Horticultural Production Development
    IDENTIFICATION OF VARIETAL POLYMORPHISM IN FICUS CARICA L. BY RAPD (RANDOMLY AMPLIFIED POLYMORPHIC DNA) MARKERS
    Authors: Y. Aka-Kaçar, A.B. Küden, M.S. Ãetiner
    Keywords: Ficus carica L., RAPDs, genotype characterization, PCR
    Abstract:
    Since 1982, the Faculty of Agriculture at the Ãukurova University has fig collection orchards containing various cultivars from Aegean, Southeast Anatolia, Marmara and Mediterranean regions. The Mediterranean area is mainly suitable for table fig production and these cultivars are named by their rind or flesh color such as yellow, green, purple, black etc. Therefore, there is confusion in identification of different cultivars. RAPD-markers allow genotype characterization to be done directly by DNA analysis. Random amplified polymorph DNA (RAPD) using twelve primers were characterized in thirty fig cultivars (Ficus carica L.). DNA was extracted from vegetative buds and amplified by PCR using random oligonucleotid primers. Genetic similarity values (Nei and Li, 1979) were calculated and UPGMA (Unweighted pair-group method analysis) cluster analysis was performed to generate a dendogram. The method, which is simple and rapid, should prove a useful system for the identification of fig cultivars.
    Full Text (PDF format, 164576 bytes)
    Citation

  • 18 years ago

    Here is some more reading thats over my head about DNA testing of figs.
    http://www.academicjournals.org/AJB/PDF/pdf2006/3Aug/Guasmi%20et%20al.pdf

  • 18 years ago

    For those curious about subject line you can search

    ficus carica dna in a search engine as i have done there is some information from Calabria, Tunisia, and maybe others on the testing they have on on fig cultivars, again its way above my head in reading this material but if its for you check it out.
    Martin

  • 18 years ago

    Well, from your first posted article:

    "Finally more works are
    necessary to enlarge the number of markers by the use
    of other molecular technologies in order to have a deeper
    insight into the molecular polymorphisms and to establish
    a varietal identification key in this crop."

    Implies that the techniques will work for typing figs but there is not enough info to establish lineages. It sounds like no one has yet estalished and genetically classified the fig varieties into a "family tree" with unique markers.

    I did not read the other articles.

  • 18 years ago

    UC Davis has used microsatellite markers to check over a hundred figs from their accessions. Somewhere online there is a dendrogram (dna relatedness "tree") that shows the relationship between varieties. I imagine the big issue is money. These analyses cost $$$ and UC Davis is not getting enough funds for what they are doing now.

  • 18 years ago

    Here's the link for that dendrogram...

    http://ucce.ucdavis.edu/files/datastore/391-301.pdf

    Look at page 6 (use tools--select and zoom)

    look at how closely related ischia black and barnisotte are by this method (and celeste and blanquette)

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