Try 'man's best friend' - duct tape. From outside & inside; stick it on & pull. Hopefully you get some out.

I use cheap Home Depot gloves for handling Opuntias, and then toss them out.. no way to get every single spine out⦠and later I always regret it when I try to wear that pair again. Only cost $2 a pair.

Trash em.

I get thick leather scraps and use that to pick up cacti. Just make sure to mark which side you a supposed to grap. Otherwise you end up with another pair of ruined gloves.

No, there's no cure, short of picking out the glochids for the next year or so, It's too bad it happened to good gloves, but you can count on a lot of company having done the same.

I use news paper, old rags and my bare hands. I am tired of ruining good gloves. I use duck tape and elmer's glue on me occasionally. I let the glue dry and pull it off, and off comes the glochids.The old magazine and rags usually too just fine. The thing is, leather is skin and needles go straight through skin. There are some cactus gloves that look like rubber gloves with a brush molded on them that work amazing , I hear. I am too cheap to splurge on them.

long handled hot dog tongs

Try duct tape

I might try to find the same gloves on Amazon. Then toss 'em (either way). Too much irritation and discomfort in a future in which you attempt to salvage.