Why are you wanting to separate them? They'll do just fine like they are- the mother will eventually die out making room for the others to fill in. If you are merely wanting to put them in different pots/areas wait until the pups are almost half the size of the mother plant before you remove them.

You didn't mention where they are on the plant. If they're in the ground, let them get some size, maybe 1/2 size of mature plant or so as directed above.
If they're up on the fruit, wait until the fruit is ripe.
kevin
p.s.cearbhaill is right in that you can leave them